Genetic modification: a tool for enhancing cellulase secretion

Land surface modification and crop diversification for enhancing productivity of a Vertisol

Vertisols occur extensively in centralIndiaand have high production potentials. Because of the high clay content (40-60% or more), high bulk density (1.5-1.8 Mg m-3) and related properties, these soils have high moisture storage capacity. Conversely, these soils become very hard when dry and very sticky when wet. Since last two decades, scientists, farmers and also the policy makers have be...

Shape engineering vs organic modification of inorganic nanoparticles as a tool for enhancing cellular internalization

In nanomedicine, physicochemical properties of the nanocarrier affect the nanoparticle's pharmacokinetics and biodistribution, which are also decisive for the passive targeting and nonspecific cellular uptake of nanoparticles. Size and surface charge are, consequently, two main determining factors in nanomedicine applications. Another important parameter which has received much less attention i...

Chemical Modification of a Cellulase from Aspergillus niger By PAUL

N-Bromosuccinimide completely inactivated the cellulase, and titration experiments showed that oxidation of one tryptophan residue per cellulase molecule coincided with 100% inactivation. CM-cellulose protected the enzyme from inactivation by N-bromosuccinimide. The cellulase was inhibited by active benzyl halides, and reaction with 2-hydroxy-5-nitrobenzyl bromide resulted in the incorporation ...

Application of Genetic Programming as a Powerful Tool for Modeling of Cellulose Acetate Membrane Preparation

Colicin A hybrids: a genetic tool for selection of type II secretion-proficient Pseudomonas strains.

The gram-negative bacterium Pseudomonas aeruginosa secretes the majority of its extracellular proteins by the type II secretion mechanism, a two-step process initiated by translocation of signal peptide-bearing exoproteins across the inner membrane. The periplasmic forms are transferred across the outer membrane by a machinery consisting of 12 xcp gene products. Although the type II secretion m...